日韩每日更新中文字幕在线观看,a三级毛片在线免费观看,亚洲国产成人情品女人久久

規(guī)格

規(guī)格	5 x 96-Well Strip Microplate Kit、2 x 96-Well Strip Microplate Kit、1 x 96-Well Strip Microplate Kit
蛋白ID	P40763
樣本類型	Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
靶標(biāo)基因	STAT3, APRF
檢測方法	Colorimetric
定量與否	Semi-Quantitative
載體	96-well Microplate
適用種屬	Human, Mouse, Rat
檢測靶標(biāo)	STAT3

詳細(xì)描述

Product Features

Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Anti-Phospho Antibody
Anti-Pan Antibody
HRP-Conjugated Secondary Antibody
Streptavidin-Conjugated HRP
Assay Diluent
TMB One-Step Substrate
Stop Solution
Lysis Buffer
Positive Control Sample

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Prepare all reagents and samples as instructed in the manual.
Add 100 μl of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μl of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μl of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

相關(guān)產(chǎn)品

Anti-Gus

Anti-Xa21

Anti-HPT

Anti-CP4-EPSPS

文件

PEL-Stat3-Y705-T.pdf

文獻(xiàn)(2)

Cornus mas L. Extract Targets the Specific Molecules of the Th17/Treg Developmental Pathway in TNBS-Induced Experimental Colitis in Rats,Tong Wang, Xiaoming Song, Hongbing Xu, Yutong Zhu, Lijuan Li, Xiaoyan Sun, Jie Chen, Beibei Liu, Qian Zhao, Yi Zhang, Ningman Yuan, Lingyan Liu, Jiakun Fang, Yunfei Xie, Shuo Liu, Rongshan Wu, Bei He, Junji Cao, Wei Huang,ENVIRONMENTAL SCIENCE & TECHNOLOGY ,(IF:11.357) 作者單位：北京大學(xué)
Species：	Rat
Sample Type：	colon

View publications

Effect of Diacerein on HOTAIR/IL-6/STAT3, Wnt/β-Catenin and TLR-4/NF-κB/TNF-α axes in colon carcinogenesis,Patrick Haider, Julia B. Kral‐Pointner, Manuel Salzmann, Florian Moik, Sonja Bleichert, Waltraud C. Schrottmaier, Christoph Kaun, Mira Brekalo, Michael B. Fischer, Walter S. Speidl, Christian Hengstenberg, Bruno K. Podesser, Kurt Huber, Ingrid Pabinger, Sylvia Knapp, Frank Brombacher, Christine Brostjan, Cihan Ay, Johann Wojta, Philipp J. Hohensinner,FASEB JOURNAL ,(IF:5.834)
Species：	Human
Sample Type：	Colorectal cancer (CRC) tumour

View publications

View all

訂購信息

可聯(lián)系當(dāng)?shù)亟?jīng)銷商進(jìn)行訂購。

聯(lián)系經(jīng)銷商

幫助

產(chǎn)品質(zhì)量保證，客戶專家支持，

點(diǎn)擊獲取更多

相關(guān)產(chǎn)品

瀏覽歷史

亚洲人成网站精品片在线_精品国产99久久久久久_麻豆精品国产自产在线观看_天堂国产一区二区三区四_日韩一区二区野外三p在线观看_中文字幕一区二区三区中文字幕_精品国产一区二区三区在线

Product Features

Application Notes

Storage/Stability