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規(guī)格

規(guī)格	1 x 96-Well Strip Microplate Kit、2 x 96-Well Strip Microplate Kit、5 x 96-Well Strip Microplate Kit
蛋白ID	P29966, P26645
樣本類(lèi)型	Adherent Cell Culture
原理方法	Cell-based
靶標(biāo)基因	MARCKS, MACS, PRKCSL
檢測(cè)方法	Colorimetric
定量與否	Semi-Quantitative
載體	96-well Microplate
適用種屬	Human, Mouse
檢測(cè)靶標(biāo)	MARCKS

詳細(xì)描述

Rapidly measure phosphorylated protein in adherent cell lines
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Kit Components

Other Materials Required

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 μl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 μl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 μl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 μl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 μl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 μl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μl of Stop Solution to each well.
Read at 450 nm immediately.